In the present study 90 previously selected hybrids based on 11 wild potato species was tested to the golden potato cyst nematode resistance. Resistant forms were identified among the hybrids that are based on 8 species: Solanum abancayense, S. alandiae, S. ambosinum, S. doddsii, S. famatinae, S. gandarillasii, S. okadae, S. vidaurrei. Occurrence of their resistance to the parasite can be accounted for coincidence of the centers of origin – an area bounded by the triangle South of Peru-Argentina-Bolivia. Valuable sources of resistance to pathotype Ro1 G. rostochiensis are species: S. alandiae, S. doddsii, S. famatinae, S. okadae. In our studies 50–70 % of the hybrids created with their participation were resistant. Species S. alandiae, S. doddsii, S. okadae are new for breeding to this feature.