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T.I. Regerand, L.V. Dubrovina.
Acidification of water as a factor of influence on water organisms lipid status stability
// Biodiversity of Fennoscandia (diversity, human impact, nature conservation). Petrozavodsk: Karelian Research Centre of RAS, 1997.
Acidification of surface waters is one of the most progressive forms of the influence on environment in Karelia and Finland. We revealed that in the condition of short-term acidification down to pH 5.5 the reaction of water invertebrates depended on the range of toleration of organisms to the pH value of water. The purpose of the present study was to innertigate the possible biochemical mechanisms like example of lipids concentrations change under the surplus of H+ ions in water environment under the 100% of organisms survivity. We pay our special attention to lipids because they are one of the main structural components of cell membranes.
The object of the experiment was molluscs Lymnaea stagnalis taken from the lake with pH=7.0. Experimental acidification was made by H2SO4 down to pH=5.5. The duration of the exposure was 48 hours. The control-CG and experimental-EG groups of organisms were in the same conditions by all other parameters. We used methods of thin-layer and gas-liquid chromatography.
It was revealed that acidification caused decrease of total phospholipids-PL and increase of triglycerides in the EG as compared with CG. Hydrolysis of PL we marked was accompanied by formation of high concentration of lyso-PL especially on the background of phosphatidylethanolamine level decrease. It was established that total content of cholin-PL increased from 61.4% in CG up to 78.9% in EG on the background of decreasing of amino-PL content. The main changes concerning fatty acids content were the redistribution of PUFA and MUFA fatty acids, which ratio increased in EG 1.7 times as compared with CG.
Adaptive processes connected with the change of the individual PL concent and their fatty acids radicals give the opportunity to keep the density of membrane and due to this - organisms survivety.
The object of the experiment was molluscs Lymnaea stagnalis taken from the lake with pH=7.0. Experimental acidification was made by H2SO4 down to pH=5.5. The duration of the exposure was 48 hours. The control-CG and experimental-EG groups of organisms were in the same conditions by all other parameters. We used methods of thin-layer and gas-liquid chromatography.
It was revealed that acidification caused decrease of total phospholipids-PL and increase of triglycerides in the EG as compared with CG. Hydrolysis of PL we marked was accompanied by formation of high concentration of lyso-PL especially on the background of phosphatidylethanolamine level decrease. It was established that total content of cholin-PL increased from 61.4% in CG up to 78.9% in EG on the background of decreasing of amino-PL content. The main changes concerning fatty acids content were the redistribution of PUFA and MUFA fatty acids, which ratio increased in EG 1.7 times as compared with CG.
Adaptive processes connected with the change of the individual PL concent and their fatty acids radicals give the opportunity to keep the density of membrane and due to this - organisms survivety.
Last modified: May 31, 2012