News
April 28, 2025
Karelian biologists ran successful trials of a technique for detecting fish infection with helminths based on traces of their DNA in water

Specialists of the Institute of Biology KarRC RAS were the first in the republic to test the method of environmental DNA analysis (eDNA) to detect a model fish parasite in an area impacted by trout farms. This is especially important in the context of a growing number of fish farms that use the practice of transporting stock (fry) from between water bodies, which creates a risk of new parasites appearing in lakes. Currently, fish have to be captured and examined to detect an infection, and for the output to be accurate the sample should be at least 15 fish. This may be problematic in the wild and costly in cage facilities. The eDNA diagnosis system can detect the presence of parasites directly in water samples.
Specialists of the Laboratory of Animal and Plant Parasitology of the Institute of Biology KarRC RAS have completed studies within the project “Parasitological monitoring of northern freshwater ecosystems by the eDNA technique” (#23-24-10073), funded by the Russian Science Foundation and the Republic of Karelia Venture Capital Fund. The scientists conducted a large experiment on isolating DNA from water samples to indicate the presence of the parasite Gyrodactylus salaris.

Gyrodactylids (Gyrodactylus salaris) are flatworms of the Monogenea class, which are shorter than 2 mm. They infect the skin and gills of fish. G. salaris is not dangerous for humans, but its high abundance indicates the fish is in poor condition and likely also carrying other pathogens: bacteria or fungi that cause infectious diseases. In such circumstances, the gyrodactylids propagate actively and become the cause of a secondary disease - gyrodactylosis. The species being quite common, it was chosen as a model object for diagnosing the occurrence of the parasite in the water area and determining the sensitivity of the eDNA method.

Currently, for G. salaris to be detected, it is necessary to collect and examine biological samples – fish skin and gills. For the output to be reliable, the sample size should be around 15-20 fish. However, there is a method in world practice which helps determine fish infection by identifying the parasite's DNA in water. In particular, it has been actively used in Norway, where G. salaris has severely affected wild populations of Atlantic salmon. The method requires specialized equipment, including tools for special water filtration, sample preparation, DNA isolation, PCR analysis and the following sequencing, as well as selection of specific genetic markers.

The first part of the work was carried out in Karelia: scientists surveyed, season-wise, 9 trout farms in lakes Onego and Ladoga, in the catchment areas of these lakes and the White Sea. In addition to examining fish in the traditional way, water samples were taken to isolate environmental DNA. In the second stage, the samples were sent for genetic analysis to Moscow, to the Russian Federal Research Institute of Fisheries and Oceanography (VNIRO), which has the equipment and technology necessary for such analysis. The results were then compared.

– The results of the study of environmental DNA prove that the parasitic impact of the fish farming facility on the water area is not limited to the fish cages but extends hundreds of meters beyond them. The eDNA diagnostic system quite accurately reveals the presence of helminths of the genus Gyrodactylus in water. This method can be used for veterinary control in the fish farming industry, – summed up Aleksey Parshukov, Candidate of Biology, Senior Researcher of the Institute of Biology KarRC RAS, the research leader.

This technique can also be applied for ecological monitoring of natural water bodies.

– With evidence of the presence of certain organisms in the water at hand, we can document the fact of anthropogenic impact. We will have the grounds to claim that the parasite is not there naturally, but is associated with trout farms in this water area, and then it will be necessary to deal with the primary source: to make the farms control the epizootic status and create conditions to prevent the pathogen from spreading, – noted Evgeny Ieshko, Doctor of Biology, Principal Researcher of the Institute of Biology KarRC RAS.


Fieldwork. Aleksey Parshukov, Senior Researcher of IB KarRC RAS, and Evgeny Ieshko, Principal Researcher of IB KarRC RAS, in the frame

However, the eDNA system cannot be immediately adopted into practice in the republic: it will take time and additional provision of equipment and reagents to complete all stages of the research cycle, starting with sample preparation and culminating in bioinformatic data processing.

Overall, the research project produced results important for basic science as well as for practical purposes. In particular, examination of the fish samples revealed a species new for the region – Gyrodactylus teuchis. It is a widespread parasite of wild and cultured salmonid fishes all over Europe. It has not been encountered in Karelian fish farms before. Here, only Gyrodactylus salaris had been known, more specifically its clone G. salaris RBT, which infects cage-reared trout.

– It is for the first time that the monogenean G. teuchis has been found on cage-reared rainbow trout in the Onego catchment or the two species found co-occurring on one host individual. Most likely, the parasite has been transported to the area with fish stock and has already dispersed widely over the region. Monogeneans G. teuchis are not pathogenic for trout in aquaculture or for wild juvenile salmon populations, but the very fact of their dispersal signals that the veterinary control of the fish stock brought over to the area is inadequate, – stated Aleksey Parshukov.

The finding has provided an opportunity to additionally study the haptoral morphology of G. teuchis. The results of the study were published in the Russian Journal of Parasitology. In particular, scientists have confirmed the correlation between the ambient temperature and the size of the hooks with which the parasites attach to the host: they are smaller in summer, because when it is warm, the monogeneans accelerate the embryogenesis rate and have no time to develop their sclerites to full size.

Photos from archives of the research participants

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